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sheep anti cd74 (R&D Systems)

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R&D Systems sheep anti cd74

a UMAP plots showing keratinocyte subclusters and sample conditions. b Bar graph showing subpopulation percentage. c Feature plot showing <t>CD74</t> expression in keratinocytes. d The percentage of CD74 + keratinocytes ( n = 3 samples for each group). e Immunohistochemistry of KRT14, CD74 in HS, normal skin of patients (NS), and lesional skin of ETR, PPR, PhR. White arrows indicate CD74 + keratinocytes. Scale bar, 50 μm. f Quantification of CD74 + keratinocytes ( n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in e ). g , h Top-ranked enriched pathways in CD74 + keratinocytes of L versus HS ( g ), L versus N ( h ). NES indicates enrichment scores. The color keys from red to blue indicate the P -value range. Two-sided permutation test without multiple comparison adjustments was used. i Heatmap of skin barrier-related genes in CD74 + keratinocytes. j Heatmap of IFN-related genes in CD74 + keratinocytes. k Immunohistochemistry of IRF1, KRT14. Scale bar, 100 μm. l Quantification of IRF1 + keratinocytes in epidermis n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in k ). m The back skins of IgG and anti-IFNγ antibody-treated mice intradermally injected with LL37 or control vehicle. Images were acquired 48 h after the first LL37 injection. Below panels, magnified images of yellow boxed areas. n The severity of the rosacea-like symptoms was determined with the redness area and score ( n = 6). o Dermal infiltrating cells were quantified ( n = 6). p The relative mRNA levels of Il1β, Il6 , Tnfα in mice skins ( n = 6). q The relative mRNA levels of Cldn4 , Cldn10 , Cldn23 in mice skins ( n = 6). r Immunohistochemistry of CLDN4, KRT14 in mice skins. Scale bar, 50 μm. Epi, Epidermis. Der, Dermis. s Quantification of relative fluorescence intensity for CLDN4 in epidermis ( n = 6). t Quantification of TEWL ( n = 6). All results are representative of at least three independent experiments. Data are presented as mean ± SEM, and P -values were determined by one-way ANOVA with Tukey’s post hoc test ( f , l , n , o , p , q , s , t ) and two-tailed unpaired (L vs HS) or paired (L vs N) Student’s t -test ( d ).

Sheep Anti Cd74, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sheep anti cd74/product/R&D Systems
Average 93 stars, based on 1 article reviews

sheep anti cd74 - by Bioz Stars, 2026-04

93/100 stars

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1) Product Images from "Single-cell transcriptomics reveals aberrant skin-resident cell populations and identifies fibroblasts as a determinant in rosacea"

Article Title: Single-cell transcriptomics reveals aberrant skin-resident cell populations and identifies fibroblasts as a determinant in rosacea

Journal: Nature Communications

doi: 10.1038/s41467-024-52946-7

a UMAP plots showing keratinocyte subclusters and sample conditions. b Bar graph showing subpopulation percentage. c Feature plot showing CD74 expression in keratinocytes. d The percentage of CD74 + keratinocytes ( n = 3 samples for each group). e Immunohistochemistry of KRT14, CD74 in HS, normal skin of patients (NS), and lesional skin of ETR, PPR, PhR. White arrows indicate CD74 + keratinocytes. Scale bar, 50 μm. f Quantification of CD74 + keratinocytes ( n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in e ). g , h Top-ranked enriched pathways in CD74 + keratinocytes of L versus HS ( g ), L versus N ( h ). NES indicates enrichment scores. The color keys from red to blue indicate the P -value range. Two-sided permutation test without multiple comparison adjustments was used. i Heatmap of skin barrier-related genes in CD74 + keratinocytes. j Heatmap of IFN-related genes in CD74 + keratinocytes. k Immunohistochemistry of IRF1, KRT14. Scale bar, 100 μm. l Quantification of IRF1 + keratinocytes in epidermis n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in k ). m The back skins of IgG and anti-IFNγ antibody-treated mice intradermally injected with LL37 or control vehicle. Images were acquired 48 h after the first LL37 injection. Below panels, magnified images of yellow boxed areas. n The severity of the rosacea-like symptoms was determined with the redness area and score ( n = 6). o Dermal infiltrating cells were quantified ( n = 6). p The relative mRNA levels of Il1β, Il6 , Tnfα in mice skins ( n = 6). q The relative mRNA levels of Cldn4 , Cldn10 , Cldn23 in mice skins ( n = 6). r Immunohistochemistry of CLDN4, KRT14 in mice skins. Scale bar, 50 μm. Epi, Epidermis. Der, Dermis. s Quantification of relative fluorescence intensity for CLDN4 in epidermis ( n = 6). t Quantification of TEWL ( n = 6). All results are representative of at least three independent experiments. Data are presented as mean ± SEM, and P -values were determined by one-way ANOVA with Tukey’s post hoc test ( f , l , n , o , p , q , s , t ) and two-tailed unpaired (L vs HS) or paired (L vs N) Student’s t -test ( d ).

Figure Legend Snippet: a UMAP plots showing keratinocyte subclusters and sample conditions. b Bar graph showing subpopulation percentage. c Feature plot showing CD74 expression in keratinocytes. d The percentage of CD74 + keratinocytes ( n = 3 samples for each group). e Immunohistochemistry of KRT14, CD74 in HS, normal skin of patients (NS), and lesional skin of ETR, PPR, PhR. White arrows indicate CD74 + keratinocytes. Scale bar, 50 μm. f Quantification of CD74 + keratinocytes ( n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in e ). g , h Top-ranked enriched pathways in CD74 + keratinocytes of L versus HS ( g ), L versus N ( h ). NES indicates enrichment scores. The color keys from red to blue indicate the P -value range. Two-sided permutation test without multiple comparison adjustments was used. i Heatmap of skin barrier-related genes in CD74 + keratinocytes. j Heatmap of IFN-related genes in CD74 + keratinocytes. k Immunohistochemistry of IRF1, KRT14. Scale bar, 100 μm. l Quantification of IRF1 + keratinocytes in epidermis n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in k ). m The back skins of IgG and anti-IFNγ antibody-treated mice intradermally injected with LL37 or control vehicle. Images were acquired 48 h after the first LL37 injection. Below panels, magnified images of yellow boxed areas. n The severity of the rosacea-like symptoms was determined with the redness area and score ( n = 6). o Dermal infiltrating cells were quantified ( n = 6). p The relative mRNA levels of Il1β, Il6 , Tnfα in mice skins ( n = 6). q The relative mRNA levels of Cldn4 , Cldn10 , Cldn23 in mice skins ( n = 6). r Immunohistochemistry of CLDN4, KRT14 in mice skins. Scale bar, 50 μm. Epi, Epidermis. Der, Dermis. s Quantification of relative fluorescence intensity for CLDN4 in epidermis ( n = 6). t Quantification of TEWL ( n = 6). All results are representative of at least three independent experiments. Data are presented as mean ± SEM, and P -values were determined by one-way ANOVA with Tukey’s post hoc test ( f , l , n , o , p , q , s , t ) and two-tailed unpaired (L vs HS) or paired (L vs N) Student’s t -test ( d ).

Techniques Used: Expressing, Immunohistochemistry, Comparison, Injection, Control, Fluorescence, Two Tailed Test

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Journal: Nature Communications

Article Title: Single-cell transcriptomics reveals aberrant skin-resident cell populations and identifies fibroblasts as a determinant in rosacea

doi: 10.1038/s41467-024-52946-7

Figure Lengend Snippet: a UMAP plots showing keratinocyte subclusters and sample conditions. b Bar graph showing subpopulation percentage. c Feature plot showing CD74 expression in keratinocytes. d The percentage of CD74 + keratinocytes ( n = 3 samples for each group). e Immunohistochemistry of KRT14, CD74 in HS, normal skin of patients (NS), and lesional skin of ETR, PPR, PhR. White arrows indicate CD74 + keratinocytes. Scale bar, 50 μm. f Quantification of CD74 + keratinocytes ( n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in e ). g , h Top-ranked enriched pathways in CD74 + keratinocytes of L versus HS ( g ), L versus N ( h ). NES indicates enrichment scores. The color keys from red to blue indicate the P -value range. Two-sided permutation test without multiple comparison adjustments was used. i Heatmap of skin barrier-related genes in CD74 + keratinocytes. j Heatmap of IFN-related genes in CD74 + keratinocytes. k Immunohistochemistry of IRF1, KRT14. Scale bar, 100 μm. l Quantification of IRF1 + keratinocytes in epidermis n = 6/6/6/6/5 samples for HS/NS/ETR/PPR/PhR group used in k ). m The back skins of IgG and anti-IFNγ antibody-treated mice intradermally injected with LL37 or control vehicle. Images were acquired 48 h after the first LL37 injection. Below panels, magnified images of yellow boxed areas. n The severity of the rosacea-like symptoms was determined with the redness area and score ( n = 6). o Dermal infiltrating cells were quantified ( n = 6). p The relative mRNA levels of Il1β, Il6 , Tnfα in mice skins ( n = 6). q The relative mRNA levels of Cldn4 , Cldn10 , Cldn23 in mice skins ( n = 6). r Immunohistochemistry of CLDN4, KRT14 in mice skins. Scale bar, 50 μm. Epi, Epidermis. Der, Dermis. s Quantification of relative fluorescence intensity for CLDN4 in epidermis ( n = 6). t Quantification of TEWL ( n = 6). All results are representative of at least three independent experiments. Data are presented as mean ± SEM, and P -values were determined by one-way ANOVA with Tukey’s post hoc test ( f , l , n , o , p , q , s , t ) and two-tailed unpaired (L vs HS) or paired (L vs N) Student’s t -test ( d ).

Article Snippet: The following primary antibodies were used: Goat anti-PDGFRA antibody (1:100, AF1062, R&D systems), Rabbit anti-PTGDS antibody (1:200, 10004344, Cayman), Rabbit anti-PDGFRA (1:500, ab203491, abcam), Goat anti-CCL19 (1:100, AF880, R&D systems), Rabbit anti-CD4 (1:500, ab183685, abcam), Rat anti-CCR7 (1:100, MAB3477, R&D systems), Rat anti-IFNγ (1:100, 14-7311-81, Thermo), Rabbit anti-MBP (1:1500, 78896, Cell signaling), Sheep anti-CD74 (1:100, AF7478, R&D systems), Rat anti-F4/80 (1:100, 14-4801-81, Thermo), Goat anti-CXCL10 antibody (1:100, AF466, R&D systems), Rabbit anti-MLC2 (1:400, 15354-1-AP, Proteintech), Mouse anti-α-SMA (1:5000, ab7817, abcam).

Techniques: Expressing, Immunohistochemistry, Comparison, Injection, Control, Fluorescence, Two Tailed Test

CD74 expression is increased in IBD patients and experimental colitis. ( A ) Increased CD74 expression in inflamed mucosa. CD74 gene expression levels in normal healthy controls, and in noninflamed and inflamed mucosa of IBD patients (n = 4, 16, and 12, respectively). Bars represent the medians. ( B and C ) Increased CD74 protein expression in epithelial cells of IBD patients. Immunohistochemical analysis of CD74 expression in mucosa of IBD patients (Crohn’s disease [CD], n = 8; ulcerative colitis [UC], n = 5) and healthy controls (n = 6). Scale bar : 50 μm. ( D and E ) Increased CD74 protein expression in epithelial cells in experimental colitis. Immunohistochemical analysis of intestinal CD74 expression in mice 24 hours after intracecal injection with 3% DSS in drinking water or normal drinking water control (n = 5 per group). Scale bar : 200 μm. ( F and G ) Increased surface CD74 expression on intestinal epithelial cells during colitis. Flow cytometry analysis of intestinal epithelial cells for CD74 surface expression (n = 6 per group). ( H ) Significant CD74 expression in crypt epithelial cells during colitis. Immunofluorescence analysis of intestinal tissue stained with anti-CD74 and EphB antibodies. Scale bar : 20 μm. One representative image of the 3 independent experiments performed is shown. Data represent means and SD. * P < .05, ** P < .01, and *** P < .001. MFI, mean fluorescent intensity.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing

doi: 10.1016/j.jcmgh.2020.01.009

Figure Lengend Snippet: CD74 expression is increased in IBD patients and experimental colitis. ( A ) Increased CD74 expression in inflamed mucosa. CD74 gene expression levels in normal healthy controls, and in noninflamed and inflamed mucosa of IBD patients (n = 4, 16, and 12, respectively). Bars represent the medians. ( B and C ) Increased CD74 protein expression in epithelial cells of IBD patients. Immunohistochemical analysis of CD74 expression in mucosa of IBD patients (Crohn’s disease [CD], n = 8; ulcerative colitis [UC], n = 5) and healthy controls (n = 6). Scale bar : 50 μm. ( D and E ) Increased CD74 protein expression in epithelial cells in experimental colitis. Immunohistochemical analysis of intestinal CD74 expression in mice 24 hours after intracecal injection with 3% DSS in drinking water or normal drinking water control (n = 5 per group). Scale bar : 200 μm. ( F and G ) Increased surface CD74 expression on intestinal epithelial cells during colitis. Flow cytometry analysis of intestinal epithelial cells for CD74 surface expression (n = 6 per group). ( H ) Significant CD74 expression in crypt epithelial cells during colitis. Immunofluorescence analysis of intestinal tissue stained with anti-CD74 and EphB antibodies. Scale bar : 20 μm. One representative image of the 3 independent experiments performed is shown. Data represent means and SD. * P < .05, ** P < .01, and *** P < .001. MFI, mean fluorescent intensity.

Article Snippet: Staining was performed using the DAKO (Santa Clara, MA) Autostainer Universal Staining System with specific antibodies: sheep anti-mouse CD74 (R&D Systems), rabbit anti-pAkt (S473; Epitomics, Burlingame, CA), rabbit anti-Ki67 (Abcam, Cambridge, MA), and mouse anti-human CD74 (LN-2; Santa Cruz).

Techniques: Expressing, Gene Expression, Immunohistochemical staining, Injection, Control, Flow Cytometry, Immunofluorescence, Staining

CD74 promotes mucosal healing in DSS- and TNBS-induced colitis. ( A – C ) CD74-deficient mice show normal intestine. Colon lengths, representative H&E-stained images, and fluorescein isothiocyanate (FITC)-dextran levels in WT ( CD74 +/+ ) and CD74-deficient mice ( CD74 –/– ) are shown (n = 8 per group). ( D – I ) CD74 promotes repair. Body weight curves, colon lengths, H&E-stained images, and histology scores of WT control and CD74 –/– mice treated with DSS followed by a recovery period. Intestinal permeability measured by FITC-dextran in serum 4 hours after gavage (n = 6 per group). ( J – M ) Body weight curves, colon lengths, H&E-stained images, and histology scores of WT control and CD74 –/– mice after TNBS-induced chronic colitis (n = 7 and 8, respectively). Scale bars : 100 μm. Data represent means and SD. * P < .05, ** P < .01, and *** P < .001.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing

doi: 10.1016/j.jcmgh.2020.01.009

Figure Lengend Snippet: CD74 promotes mucosal healing in DSS- and TNBS-induced colitis. ( A – C ) CD74-deficient mice show normal intestine. Colon lengths, representative H&E-stained images, and fluorescein isothiocyanate (FITC)-dextran levels in WT ( CD74 +/+ ) and CD74-deficient mice ( CD74 –/– ) are shown (n = 8 per group). ( D – I ) CD74 promotes repair. Body weight curves, colon lengths, H&E-stained images, and histology scores of WT control and CD74 –/– mice treated with DSS followed by a recovery period. Intestinal permeability measured by FITC-dextran in serum 4 hours after gavage (n = 6 per group). ( J – M ) Body weight curves, colon lengths, H&E-stained images, and histology scores of WT control and CD74 –/– mice after TNBS-induced chronic colitis (n = 7 and 8, respectively). Scale bars : 100 μm. Data represent means and SD. * P < .05, ** P < .01, and *** P < .001.

Techniques: Staining, Control, Permeability

CD74 promotes mucosal healing in amebic colitis. ( A and B ) Increased CD74 protein expression in epithelial cells in human amebic colitis. Immunohistochemical analysis of CD74 expression in mucosa of patients with amebic colitis and normal healthy controls (n = 3 and 6, respectively). ( C – E ) H&E-stained images and histology scores of WT control and CD74 –/– mice after infection with E histolytica . Intestinal permeability measured by albumin levels in cecal luminal contents (n = 7 per group). ( F and G ) H&E-stained images, histology scores, and albumin levels in WT mice treated with anti-CD74 or control antibodies (n = 8 per group). Scale bars : 200 μm. Data represent means and SD. * P < .05, ** P < .01, *** P < .001.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing

doi: 10.1016/j.jcmgh.2020.01.009

Figure Lengend Snippet: CD74 promotes mucosal healing in amebic colitis. ( A and B ) Increased CD74 protein expression in epithelial cells in human amebic colitis. Immunohistochemical analysis of CD74 expression in mucosa of patients with amebic colitis and normal healthy controls (n = 3 and 6, respectively). ( C – E ) H&E-stained images and histology scores of WT control and CD74 –/– mice after infection with E histolytica . Intestinal permeability measured by albumin levels in cecal luminal contents (n = 7 per group). ( F and G ) H&E-stained images, histology scores, and albumin levels in WT mice treated with anti-CD74 or control antibodies (n = 8 per group). Scale bars : 200 μm. Data represent means and SD. * P < .05, ** P < .01, *** P < .001.

Techniques: Expressing, Immunohistochemical staining, Staining, Control, Infection, Permeability

CD74 signaling activates the pro-proliferative pathways. ( A ) Confocal immunofluorescence images of intestinal epithelial cells of IBD patient and normal healthy controls expressing MIF. Scale bars : 50 μm. ( B and C ) Increased MIF secretion in colitis. Intestinal tissue and luminal MIF levels in mice 24 hours after intracecal injection with 3% DSS in drinking water or normal drinking water control (n = 6 per group). ( D ) Genotyping and immunoblot analysis of CD74 +/+ and CD74 –/– intestinal epithelial cells. ( E and F ) CD74- MIF interaction activates Akt and ERK pathways. Immunoblot analysis of Akt and ERK phosphorylation in CD74 +/+ and CD74 –/– intestinal epithelial cells stimulated with MIF. Akt and ERK phosphorylation assessed by immunoblot analysis of intestinal epithelial cells stimulated with MIF in the presence of anti-CD74 or control antibodies. Actin served as a loading control. ( G ) Immunohistochemical analysis of Akt phosphorylation in epithelial cells of CD74 +/+ and CD74 –/– mice during ( H ) steady-state control and ( I ) colitis conditions (n = 5 per group). ( J ) Intestinal tissue stained for Ki67 by immunohistochemistry and quantitated. Scale bars : 50 μm. ( K – M ) CD74 stimulation promotes epithelial cell proliferation and wound closure. Percentage proliferation of CD74 +/+ and CD74 –/– intestinal epithelial cells 24 hours after dose-dependent stimulation with MIF. Representative images and quantitation of CD74 +/+ and CD74 –/– intestinal epithelial cell wound closure at baseline (T0) and after 6 hours (T6) and 24 hours (T24) of MIF (100 ng/mL) stimulation. Data are representative of at least 2 independent experiments. Data represent means and SD. *P < .05,** P < .01. Ab, antibody; EpCAM, epithelial cell adhesion molecule; P-Akt, phosphorylated Akt; P-ERK, phosphorylated ERK.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing

doi: 10.1016/j.jcmgh.2020.01.009

Figure Lengend Snippet: CD74 signaling activates the pro-proliferative pathways. ( A ) Confocal immunofluorescence images of intestinal epithelial cells of IBD patient and normal healthy controls expressing MIF. Scale bars : 50 μm. ( B and C ) Increased MIF secretion in colitis. Intestinal tissue and luminal MIF levels in mice 24 hours after intracecal injection with 3% DSS in drinking water or normal drinking water control (n = 6 per group). ( D ) Genotyping and immunoblot analysis of CD74 +/+ and CD74 –/– intestinal epithelial cells. ( E and F ) CD74- MIF interaction activates Akt and ERK pathways. Immunoblot analysis of Akt and ERK phosphorylation in CD74 +/+ and CD74 –/– intestinal epithelial cells stimulated with MIF. Akt and ERK phosphorylation assessed by immunoblot analysis of intestinal epithelial cells stimulated with MIF in the presence of anti-CD74 or control antibodies. Actin served as a loading control. ( G ) Immunohistochemical analysis of Akt phosphorylation in epithelial cells of CD74 +/+ and CD74 –/– mice during ( H ) steady-state control and ( I ) colitis conditions (n = 5 per group). ( J ) Intestinal tissue stained for Ki67 by immunohistochemistry and quantitated. Scale bars : 50 μm. ( K – M ) CD74 stimulation promotes epithelial cell proliferation and wound closure. Percentage proliferation of CD74 +/+ and CD74 –/– intestinal epithelial cells 24 hours after dose-dependent stimulation with MIF. Representative images and quantitation of CD74 +/+ and CD74 –/– intestinal epithelial cell wound closure at baseline (T0) and after 6 hours (T6) and 24 hours (T24) of MIF (100 ng/mL) stimulation. Data are representative of at least 2 independent experiments. Data represent means and SD. *P < .05,** P < .01. Ab, antibody; EpCAM, epithelial cell adhesion molecule; P-Akt, phosphorylated Akt; P-ERK, phosphorylated ERK.

Techniques: Immunofluorescence, Expressing, Injection, Control, Western Blot, Phospho-proteomics, Immunohistochemical staining, Staining, Immunohistochemistry, Quantitation Assay

Role of CD74 signaling in immune cells. ( A ) Flow cytometry analysis of immune cells for CD74 surface expression after 3% DSS in drinking water or normal drinking water control (n = 5 per group). ( B ) Colon lengths, ( C ) histology scores, and ( D ) fluorescein isothiocyanate (FITC)-dextran levels of mice with CD74 +/+ and CD74 –/– bone marrow (BM) treated with DSS followed by a recovery period (n = 6 per group). Data represent means and SD. MFI, mean fluorescent intensity.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing

doi: 10.1016/j.jcmgh.2020.01.009

Figure Lengend Snippet: Role of CD74 signaling in immune cells. ( A ) Flow cytometry analysis of immune cells for CD74 surface expression after 3% DSS in drinking water or normal drinking water control (n = 5 per group). ( B ) Colon lengths, ( C ) histology scores, and ( D ) fluorescein isothiocyanate (FITC)-dextran levels of mice with CD74 +/+ and CD74 –/– bone marrow (BM) treated with DSS followed by a recovery period (n = 6 per group). Data represent means and SD. MFI, mean fluorescent intensity.

Techniques: Flow Cytometry, Expressing, Control

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1) Product Images from "Single-cell transcriptomics reveals aberrant skin-resident cell populations and identifies fibroblasts as a determinant in rosacea"

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